"There is zero tolerance on doping related issues," said Khairy who was responding to the Court of Arbitration for Sport's (CAS) 10-year ban on former national athletics coach Harun Rasheed yesterday.
"The ministry and the National Sports Council (NSC) will never compromise on doping cases.
"If any of our athletes fail to turn up for a scheduled doping test and then get punished, they will get no sympathy from us," said Khairy yesterday.
The minister met with NSI head Ahmad Shapawi Ismail in Bukit Jalil yesterday.
On May 24, Harun was found guilty of instructing six sprinters not to give their urine samples to the National Sports Institute (NSI) two years ago.
Siti Zubaidah Adabi, Siti Fatimah Mohamed, Norjannah Hafiszah Kamaruddin, Yee Yi Leng, Nurul Sarah Kadir and Noor Imran Hadi were also found guilty.
Except for Siti Fatimah, who was suspended for 18 months, the others were banned for two years.
Khairy also touched on the importance of applying sports science to training.
"Coaches and athletes must adopt sports science methods to prepare for high-level events. I am disappointed as the response has not been up to a desired level.
"In modern sports, nutrition, conditioning and mental training are very important. According to NSI, World No 1 Lee Chong Wei is among the few who religiously follows modern training methods," said Khairy.
The ministry also hopes squash will become a medal sport at the 2020 Olympics.
"Now that squash has been shortlisted together with wrestling and baseball, everyone must work harder to make sure the sport will make it to the end.
"I believe wrestling will be our main contender, so it will be tough for sure."
The International Olympic Committee shortlisted the three sports from a list of eight in Russia last week.